Article
Segment IV of a Salmonella flagellin gene specifies flagellar antigen epitopes.
Molecular microbiology - 1 Feb 1991
Newton S M, Wasley R D, Wilson A, Rosenberg L T, Miller J F, Stocker B A
Abstract excerpt
Each of the two mutants isolated from a fliC (= hag, flagellin-deficient) Escherichia coli strain made motile by a plasmid carrying the fliC gene of Salmonella muenchen by selection for motility in the presence of anti-d (Salmonella flagellar antigen) serum had both lost and gained one or more subfactors of the wild-type antigen. In one mutant codon 246 was GAC (alanine) instead of GCC (asparagine); the other had...
Topics
- Antigens, Bacterial
- Blotting, Western
- Cloning, Molecular
- Epitopes
- Flagella
- Flagellin
- Mutation
- Plasmids
- Restriction Mapping
- Salmonella