Article
Identification and genetic mapping of highly polymorphic microsatellite loci from an EST database of the septoria tritici blotch pathogen Mycosphaerella graminicola.
Fungal genetics and biology : FG & B - 1 May 2007
Goodwin Stephen B, van der Lee Theo A J, Cavaletto Jessica R, Te Lintel Hekkert Bas, Crane Charles F, Kema Gert H J
Abstract excerpt
A database of 30,137 EST sequences from Mycosphaerella graminicola, the septoria tritici blotch fungus of wheat, was scanned with a custom software pipeline for di- and trinucleotide units repeated tandemly six or more times. The bioinformatics analysis identified 109 putative SSR loci, and for 99 of them, flanking primers were developed successfully and tested for amplification and polymorphism by PCR on five...
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