Article
Expression and functional study of wild-type and mutant human cytochrome P450c21 in Saccharomyces cerevisiae.
DNA and cell biology - 1 Apr 1991
Wu D A, Hu M C, Chung B C
Abstract excerpt
The most common cause of congenital adrenal hyperplasia is deficiency of cytochrome P450c21 (21-hydroxylase), which catalyzes the synthesis of adrenal steroids. We have cloned the human P450c21 cDNA into yeast expression vectors under the control of either the glyceraldehyde-3-phosphate-dehydroge...
Topics
- Blotting, Northern
- Blotting, Western
- Gene Expression Regulation, Fungal
- Genes, Fungal
- Humans
- Kinetics
- Microsomes
- Mutation
- Plasmids
- RNA
- Saccharomyces cerevisiae
- Spectrum Analysis
- Steroid 21-Hydroxylase