Article
Long-term room temperature storage of high-quality embryonic stem cell genomic DNA extracted with a simple and rapid procedure.
Journal of biomolecular techniques : JBT - 1 Sept 2006
Tribioli Carla, Lufkin Thomas
Abstract excerpt
A very simple procedure for the isolation of high-quality, high-molecular-weight genomic DNA from embryonic stem cells is described. The DNA is very stable once dried and can be stored for long periods of time without refrigeration. Living cells are lysed in a sodium dodecyl sulfate and EDTA buffer containing proteinase K and then air-dried. Samples can be processed in bulk, and an individual can easily process...
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