Determination of Escherichia coli O types by allele-specific polymerase chain reaction: application to the O types involved in human septicemia.

Abstract excerpt

Escherichia coli can be serotyped by determination of somatic (O), capsular (K), and flagellar (H) antigens, and clear associations exist between specific O antigens and pathogenic behavior. However, E. coli is very challenging to O type with traditional serologic methods, making new methods for E. coli somatic antigen detection highly desirable. Here, we describe a simple alternative molecular method for...

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