Establishment of A Competitive ELISA (cELISA) System for the Detection of Influenza A Virus Nucleoprotein Antibodies and its Application to Field Sera from Different Species

Abstract excerpt

A recombinant baculovirus (RBV) encoding the nucleoprotein (NP) of avian influenza virus (AIV) was generated and the appropriate protein was expressed in Sf9 cells. Purified recombinant NP and the NP-specific monoclonal antibody HB65 were used to establish a competitive ELISA (cELISA) system for the detection of NP-specific antibodies in sera of ducks, geese and wild birds. Tests to evaluate this method were...

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