Article
The potassium channel KAT1 is activated by plant and animal 14-3-3 proteins.
The Journal of biological chemistry - 24 Nov 2006
Sottocornola Barbara, Visconti Sabina, Orsi Sara, Gazzarrini Sabrina, Giacometti Sonia, Olivari Claudio, Camoni Lorenzo, Aducci Patrizia, Marra Mauro, Abenavoli Alessandra, Thiel Gerhard, Moroni Anna
Abstract excerpt
14-3-3 proteins modulate the plant inward rectifier K+ channel KAT1 heterologously expressed in Xenopus oocytes. Injection of recombinant plant 14-3-3 proteins into oocytes shifted the activation curve of KAT1 by +11 mV and increased the tau(on). KAT1 was also modulated by 14-3-3 proteins of Xenopus oocytes. Titration of the endogenous 14-3-3 proteins by injection of the peptide Raf 621p resulted in a strong...
Topics
- 14-3-3 Proteins
- Animals
- Arabidopsis Proteins
- Cell Membrane
- Cesium
- Electrophysiology
- Escherichia coli
- Ion Channel Gating
- Mutation
- Oocytes
- Patch-Clamp Techniques
- Potassium Channels, Inwardly Rectifying
- Recombinant Proteins