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Article

Standardization of sample preparation, staining and sampling methods for automated sperm head morphometry analysis of boar spermatozoa

25 Apr 2006

Abstract excerpt

The accuracy of computer-assisted sperm morphometry analysis (CASMA) depends on the careful preparation, fixation and staining of spermatozoa. The efficiency of CASMA may be enhanced by developing optimized protocols. The aim of the present study was to evaluate the influence of sperm washing and the use of three staining techniques [rapid Panoptic, Hemacolor and Harris's Haematoxylin (HH)] on image-processing...

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PMID: 16968497
DOI: 10.1111/j.1365-2605.2006.00696.x

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