Article
Development of a rapid strain differentiation method for methicillin-resistant Staphylococcus aureus isolated in Japan by detecting phage-derived open-reading frames.
Journal of applied microbiology - 1 Oct 2006
Suzuki M, Tawada Y, Kato M, Hori H, Mamiya N, Hayashi Y, Nakano M, Fukushima R, Katai A, Tanaka T, Hata M, Matsumoto M, Takahashi M, Sakae K
Abstract excerpt
AIMS: To develop a rapid genotyping method for investigating outbreaks of methicillin-resistant strains of Staphylococcus aureus (MRSA) isolated in Japan. METHODS AND RESULTS: Isolates were genotyped by detecting the keeping pattern of 16 open-reading frames (ORFs), a process we call phage ORF typing (POT). Thirteen of the ORFs were selected from phage genomes and one from a genomic island SaGIm in the genome of...
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