Article
Detection of point mutations in type I collagen by RNase digestion of RNA/RNA hybrids.
Nucleic acids research - 25 Jul 1990
Grange D K, Gottesman G S, Lewis M B, Marini J C
Abstract excerpt
We have developed a strategy for the detection, localization and sequence determination of point mutations in the mRNA coding for the alpha 1(I) and alpha 2(I) chains of type I collagen. Point mutations are detected by RNase A cleavage of mismatches in RNA/RNA hybrids. The mRNAs coding for the fi...
Topics
- Alleles
- Base Composition
- Base Sequence
- Cloning, Molecular
- Collagen
- Cyanogen Bromide
- Humans
- Molecular Sequence Data
- Mutation
- Nucleic Acid Hybridization
- Osteogenesis Imperfecta
- Polymerase Chain Reaction
- RNA
- RNA, Antisense
- RNA, Messenger
- Repetitive Sequences, Nucleic Acid
- Restriction Mapping
- Ribonucleases