Article
Identification of type I collagen gene polymorphisms: tolerance of sequence variation at an alpha 2(I) helix Y position.
Matrix biology : journal of the International Society for Matrix Biology - 1 Jan 1994
Sztrolovics R, van der Rest M, Roughley P J
Abstract excerpt
This study has examined the frequency and distribution of polymorphisms in the type I collagen coding sequences. RNA from a group of human skin fibroblast cell lines was analyzed by the chemical cleavage mismatch detection method using hydroxylamine, a reagent specific for C-base mismatches, and...
Topics
- Amino Acid Sequence
- Base Sequence
- Cells, Cultured
- Collagen
- DNA Primers
- DNA Probes
- DNA, Complementary
- Fibroblasts
- Genetic Variation
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Procollagen
- Protein Precursors
- Protein Structure, Secondary
- Skin