Article
Mutational and functional analysis of the beta-carotene ketolase involved in the production of canthaxanthin and astaxanthin.
Applied and environmental microbiology - 1 Sept 2006
Ye Rick W, Stead Kristen J, Yao Henry, He Hongxian
Abstract excerpt
Biosynthesis of the commercial carotenoids canthaxanthin and astaxanthin requires beta-carotene ketolase. The functional importance of the conserved amino acid residues of this enzyme from Paracoccus sp. strain N81106 (formerly classified as Agrobacterium aurantiacum) was analyzed by alanine-scanning mutagenesis. Mutations in the three highly conserved histidine motifs involved in iron coordination abolished its...
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