Article
A mutational analysis of the epitopes of recombinant human H-ferritin.
Biochimica et biophysica acta - 19 Jun 1990
Arosio P, Cozzi A, Ingrassia R, Levi S, Luzzago A, Ruggeri G, Iacobello C, Santambrogio P, Albertini A
Abstract excerpt
Murine monoclonal antibodies were elicited by the recombinant human H-ferritin overexpressed in Escherichia coli. They had a specificity analogous to that of the antibodies elicited by natural human H-chain, and all of them showed low additivity in binding the recombinant ferritin. Four antibodies of each group were challenged with four H-ferritin mutants overexpressed in E. coli, altered in different accessible...
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