Article
Multilocus sequence typing system for the endosymbiont Wolbachia pipientis.
Applied and environmental microbiology - 1 Nov 2006
Baldo Laura, Dunning Hotopp Julie C, Jolley Keith A, Bordenstein Seth R, Biber Sarah A, Choudhury Rhitoban Ray, Hayashi Cheryl, Maiden Martin C J, Tettelin Hervè, Werren John H
Abstract excerpt
The eubacterial genus Wolbachia comprises one of the most abundant groups of obligate intracellular bacteria, and it has a host range that spans the phyla Arthropoda and Nematoda. Here we developed a multilocus sequence typing (MLST) scheme as a universal genotyping tool for Wolbachia. Internal fragments of five ubiquitous genes (gatB, coxA, hcpA, fbpA, and ftsZ) were chosen, and primers that amplified across the...
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