Article
Usefulness of ligation-mediated PCR genotyping in tracking outbreak-associated Mycobacterium tuberculosis strains.
Archives de l'Institut Pasteur de Tunis - 1 Jan 2005
Mardassi H, Namouchi A, Karbouli A, Khabouchi N, Haltiti R, Zarrouk M, Mhennii B, Kaabi S
Abstract excerpt
With the emergence of a multidrug resistant tuberculosis (MDR-TB) outbreak, the availability of a rapid typing method to carry out a nationwide prospective survey for the tracking of newly emerging MDR-TB foci became a priority. For this purpose, we have applied the IS6110 PCR-based genotyping assay, namely, LM-PCR (ligation-mediated PCR). The latter relies on ligation of a synthetic oligonucleotide priming site...
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