Article
Identification, cloning and characterization of cysK, the gene encoding O-acetylserine (thiol)-lyase from Azospirillum brasilense, which is involved in tellurite resistance.
FEMS microbiology letters - 1 Aug 2006
Ramírez Alberto, Castañeda Miguel, Xiqui María L, Sosa Araceli, Baca Beatriz E
Abstract excerpt
O-Acetylserine (thiol)-lyase (cysteine synthase) was purified from Azospirillum brasilense Sp7. After hydrolysis of the purified protein, amino acid sequences of five peptides were obtained, which permitted the cloning and sequencing of the cysK gene. The deduced amino acid sequence of cysteine synthase exhibited homology with several putative proteins from Alpha- and Gammaproteobacteria. Azospirillum brasilense...
Topics
- Azospirillum brasilense
- Base Sequence
- Carbon-Oxygen Lyases
- Chromatography
- Cloning, Molecular
- Drug Resistance, Fungal
- Genetic Complementation Test
- Mutation
- Oxidation-Reduction
- Phenotype
- Tellurium