Article
Phenotypic mixing test to detect and assay avian leukosis viruses.
Avian diseases - 1 Jan 2000
Okazaki W, Purchase H G, Burmester B R
Abstract excerpt
A phenotypic mixing (PM) test for detecting and assaying avian leukosis viruses (ALV) of the A, B, C, and D subgroups is described. An ALV and Rous sarcoma virus RSV-0) are phenotypically mixed by co-cultivating on C/O (cells susceptible to all subgroups of ALV) cells for a certain period. Then the RSV with the new virus property is assayed on C/E cells (cells resistant to infection with subgroup E...
Topics
- Animals
- Avian Leukosis
- Avian Leukosis Virus
- Blood
- Cells, Cultured
- Chick Embryo
- Chickens
- Complement Fixation Tests
- Culture Media
- Fibroblasts
- Phenotype
- Temperature
