Article
Effects of genetic polymorphisms at the growth hormone gene on milk yield in Serra da Estrela sheep.
The Journal of dairy research - 1 Nov 2006
Marques Maria do Rosário, Santos Ingrid C, Carolino Nuno, Belo Carlos C, Renaville Robert, Cravador Alfredo
Abstract excerpt
The five exons and the 5' and 3'-untranslated regions (5'-UTR and 3'-UTR) of the oGH gene were screened for mutations using PCR-single strand conformation polymorphism (PCR-SSCP) procedures in 523 Serra da Estrela ewes and were found to be highly polymorphic. The region extending across and between the GH2-N and GH2-Z copies was sequenced allowing the design of primers for the specific PCR amplification of each...
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