Article
Conversion of Lactococcus lactis cell envelope proteinase specificity by partial allele exchange.
Journal of applied microbiology - 1 Jun 2006
Broadbent J R, Rodríguez B T, Joseph P, Smith E A, Steele J L
Abstract excerpt
AIMS: To determine whether conversion of lactocepin substrate binding regions by gene replacement can alter lactocepin specificity in Lactococcus lactis starter bacteria without affecting other important strain properties. METHODS AND RESULTS: We utilized two-step gene replacement to convert substrate-binding determinants in the L. lactis prtP genes encoding group h (bitter) lactocepin in two industrial strains...
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