Article
Photosynthetic electron transport in genetically altered photosystem II reaction centers of chloroplasts.
Proceedings of the National Academy of Sciences of the United States of America - 15 Oct 1991
Roffey R A, Golbeck J H, Hille C R, Sayre R T
Abstract excerpt
Using a cotransformation system to identify chloroplast transformants in Chlamydomonas reinhardtii, we converted histidine-195 of the photosystem II reaction center D1 protein to a tyrosine residue. The mutants were characterized by a reduced quantum efficiency for photosynthetic oxygen evolution, which varied in a pH-dependent manner, a reduced capacity to oxidize artificial donors to photosystem II, and P680+...
Topics
- Animals
- Chlamydomonas
- Chloroplasts
- DNA
- Electron Spin Resonance Spectroscopy
- Electron Transport
- Genome
- Kinetics
- Light
- Light-Harvesting Protein Complexes
- Mutagenesis, Site-Directed
