Article
Molecular characterization of pestiviruses isolated from bovines in Chile.
Veterinary microbiology - 15 Jun 2006
Pizarro-Lucero José, Celedón María-Orfelia, Aguilera Marcela, de Calisto Alejandra
Abstract excerpt
Thirty-three pestiviruses isolated from bovines on different farms in Chile were characterized at the molecular level. The 5'-untranslated region (5'UTR) of the isolates was amplified by reverse transcription-polymerase chain reaction (RT-PCR) and subsequently digested with restriction enzymes (RE) Bgl I, Xho I and Pst I. Furthermore, the isolates were amplified by differential RT-PCR, which selectively amplified...
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