Article
Genotyping of Ochrobactrum anthropi by recA-based comparative sequence, PCR-RFLP, and 16S rRNA gene analysis.
FEMS microbiology letters - 1 Apr 2006
Scholz Holger C, Tomaso Herbert, Al Dahouk Sascha, Witte Angela, Schloter Michael, Kämpfer Peter, Falsen Enevold, Neubauer Heinrich
Abstract excerpt
A recA-PCR restriction fragment length polymorphism assay was developed to study intraspecies variation among Ochrobactrum anthropi. Primers deduced from the known recA gene sequence of the genetically closely related genus Brucella allowed the specific amplification of a 1065 bp recA fragment fr...
Topics
- Animals
- Bacterial Typing Techniques
- Cattle
- Dogs
- Genes, rRNA
- Genotype
- Humans
- Molecular Sequence Data
- Ochrobactrum
- Ochrobactrum anthropi
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
