Article
[Quantification and phenotypic analysis of hCMV specific CTL in peripheral blood from HLA-A2+ donors].
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology - 1 Mar 2006
Zha Qing-bing, He Xian-hui, Xu Li-hui, Chi Xiao-yun, Zeng Yao-ying
Abstract excerpt
AIM: To optimize tetramer staining condition using HLA-A*0201 tetramer (A2-NLV tetramer) loaded with NLV peptide (pp65(495-503)) derived from structural protein pp65 of human cytomegalovirus and to investigate its application in phenotyping of specific cytotoxic T lymphocytes (CTL). METHODS: Peripheral blood from HLA-A2(+) donors was first stained with A2-NLV tetramer/PE under different conditions and then...
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