Article
Nitrogen-fixing sinorhizobia with Medicago laciniata constitute a novel biovar (bv. medicaginis) of S. meliloti.
Systematic and applied microbiology - 1 Nov 2006
Villegas Maria Del Carmen, Rome Sophie, Mauré Lucette, Domergue Odile, Gardan Louis, Bailly Xavier, Cleyet-Marel Jean-Claude, Brunel Brigitte
Abstract excerpt
Sixty-eight new rhizobial isolates were obtained from root-nodules of Medicago laciniata and from Mediterranean soils in Tunisia and France. All of them were identified as Sinorhizobium meliloti on the basis of PCR-RFLP analyses of 16S rDNA and the intergenic spacer sequence between 16S and 23S rDNAs. DNA/DNA hybridization, phenotypic characterization and 16S rRNA gene sequencing led to the conclusion that they...
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