Article
Transposome mutagenesis of an integral membrane transporter in Corynebacterium matruchotii.
Biochemical and biophysical research communications - 17 Feb 2006
Wang Cindy, Hayes Barry, Vestling Martha M, Takayama Kuni
Abstract excerpt
A transposon-5 insertion library of Corynebacterium matruchotii ATCC14266 was generated and screened for mutants with altered corynomycolic acid content. One of these designated 319 mutants showed an interruption of a gene encoding an integral membrane protein. MALDI mass spectra of trehalose monocorynomycolate (TMCM), trehalose dicorynomycolate, and methyl corynomycolates derived from cell wall...
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