Article
Effect of mutations K97A and E128A on RNA binding and self assembly of papaya mosaic potexvirus coat protein.
The FEBS journal - 1 Jan 2006
Tremblay Marie-Hélène, Majeau Nathalie, Gagné Marie-Eve Laliberté, Lecours Katia, Morin Hélène, Duvignaud Jean-Baptiste, Bolduc Marilène, Chouinard Nicolas, Paré Christine, Gagné Stéphane, Leclerc Denis
Abstract excerpt
Papaya mosaic potexvirus (PapMV) coat protein (CP) was expressed (CPdeltaN5) in Escherichia coli and showed to self assemble into nucleocapsid like particles (NLPs). Twenty per cent of the purified protein was found as NLPs of 50 nm in length and 80% was found as a multimer of 450 kDa (20 subunits) arranged in a disk. Two mutants in the RNA binding domain of the PapMV CP, K97A and E128A showed interesting...
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