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Article

Comparative sequence analysis of the internal transcribed spacer 1 of Ochrobactrum species.

Systematic and applied microbiology - 1 Jun 2006

Lebuhn Michael, Bathe Stephan, Achouak Wafa, Hartmann Anton, Heulin Thierry, Schloter Michael

Abstract excerpt

The internal 16S/23S rDNA (rrs/rrl) internal spacer region 1 (ITS1) of 54 Ochrobactrum strains and close relatives was analysed. Separation of ITS1 containing PCR products by gel-electrophoresis, DGGE, cloning and sequencing revealed ITS1 length and sequence heterogeneity. We found up to 5 different allelic ITS1 stretches within a single strain (Ochrobactrum intermedium LMG 3301T), and 2-3 different ITS1 alleles...

Topics

Alleles
DNA, Bacterial
DNA, Intergenic
DNA, Ribosomal
Gene Transfer, Horizontal
Genes, rRNA
Molecular Sequence Data
Nucleic Acid Hybridization
Ochrobactrum
Phylogeny
Polymorphism, Genetic

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

RNA, Ribosomal, 16S

Sequence Analysis, DNA

Statistics as Topic

Source

Identifiers

PMID: 16352412
DOI: 10.1016/j.syapm.2005.11.003

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-16352412-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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