Article
Direct amplification of rhizobial nodC sequences from soil total DNA and comparison to nodC diversity of root nodule isolates.
FEMS microbiology ecology - 1 Sept 2005
Sarita Sarita, Sharma Parveen K, Priefer Ursula B, Prell Juergen
Abstract excerpt
A group-specific primer set was developed using nodC as a target gene for the amplification of rhizobial sequence diversity from nodule isolates and total soil DNA preparations. The primer set was tested on 209 nodule isolates, recovered from six different trap plant species which were grown in two soil samples collected from a chickpea and a wheat field site in India. We also amplified and cloned PCR products...
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