Article
Direct amplification of nodD from community DNA reveals the genetic diversity of Rhizobium leguminosarum in soil.
Environmental microbiology - 1 Jun 2001
Zézé A, Mutch L A, Young J P
Abstract excerpt
Sequences of nodD, a gene found only in rhizobia, were amplified from total community DNA isolated from a pasture soil. The polymerase chain reaction (PCR) primers used, Y5 and Y6, match nodD from Rhizobium leguminosarum biovar trifolii, R. leguminosarum biovar viciae and Sinorhizobium meliloti. The PCR product was cloned and yielded 68 clones that were identified by restriction pattern as derived from biovar...
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