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Detection of Canine Distemper Virus in Blood Samples by Reverse Transcription Loop‐Mediated Isothermal Amplification

1 Nov 2005

Abstract excerpt

Reverse transcription loop-mediated isothermal amplification (RT-LAMP) was used to detect canine distemper virus (CDV) genomic RNA. A set of four primers, two outer and two inner, were designed from CDV genomic RNA targeting the nucleocapsid protein gene. The optimal reaction time and temperature for LAMP were determined to be 60 min at 65 degrees C. The relative sensitivity and specificity of RT-LAMP was found...

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PMID: 16283921
DOI: 10.1111/j.1439-0450.2005.00886.x

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