DETECTION AND DIFFERENTIATION OF FILARIAL PARASITES BY UNIVERSAL PRIMERS AND POLYMERASE CHAIN REACTION–RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS

Abstract excerpt

Filarial nematode parasites are a serious cause of morbidity in humans and animals. Identification of filarial infection using traditional morphologic criteria can be difficult and lead to misdiagnosis. We report on a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)-based method to detect and differentiate a broad range of filarial species in a single PCR. The first internal...

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