16S rRNA Gene-Based Identification of Midgut Bacteria from Field-Caught <i>Anopheles gambiae</i> Sensu Lato and <i>A. funestus</i> Mosquitoes Reveals New Species Related to Known Insect Symbionts

Abstract excerpt

Field-collected mosquitoes of the two main malaria vectors in Africa, Anopheles gambiae sensu lato and Anopheles funestus, were screened for their midgut bacterial contents. The midgut from each blood-fed mosquito was screened with two different detection pathways, one culture independent and one culture dependent. Bacterial species determination was achieved by sequence analysis of 16S rRNA genes. Altogether, 16...

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