Article
Polyphenol oxidase activity expression in Ralstonia solanacearum.
Applied and environmental microbiology - 1 Nov 2005
Hernández-Romero Diana, Solano Francisco, Sanchez-Amat Antonio
Abstract excerpt
Sequencing of the genome of Ralstonia solanacearum revealed several genes that putatively code for polyphenol oxidases (PPOs). To study the actual expression of these genes, we looked for and detected all kinds of PPO activities, including laccase, cresolase, and catechol oxidase activities, in cellular extracts of this microorganism. The conditions for the PPO assays were optimized for the phenolic substrate,...
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