Article
[Cellular phenotype conversion induced by co-culture of human mesenchymal stem cells cocultured with human sweat gland cells].
Zhonghua yi xue za zhi - 20 Jul 2005
Li Hai-hong, Fu Xiao-bing, Zhou Gang, Fei Pei, Chen Wei, Bai Xiao-dong, Cai Cun-liang, Sun Tong-zhu
Abstract excerpt
OBJECTIVE: To investigate the cellular phenotype conversion during human mesenchymal stem cells (hMSCs) cocultured with injured human sweat gland cells (hSGCs) in vitro. METHODS: HMSCs and hSGCs were isolated and cultured and expanded respectively. The antigens expression of hMSCs and hSGCs were detected by two-steps immunocytochemistry. HMSCs were labeled with BrdU. The hSGCs were heat-shocked at 47 degrees C...
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