Article
[Cloning and expression of P450 genes CYPA14 and CYPA16 from Candida tropicalis 1230].
Wei sheng wu xue bao = Acta microbiologica Sinica - 1 Aug 2005
He Feng, Chen Yuan-Tong
Abstract excerpt
Cytochrome P450 (CYP) as a monooxygenase catalyzes the limiting-rate reaction in the oxidation pathway of Candida tropicalis (C. tropicalis). Two members of P450 gene CYPA16 and CYPA14 were isolated from C. tropicalis 1230 by cassette PCR method, based on cloning of the P450 partial gene. They are tandemly arranged on the chromosome, encoding the gene products of 522 and 540 amino acid residues respectively. The...
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