Article
Identification of novel binding partners for tuberous sclerosis complex 2 (TSC2) by yeast two-hybrid approach.
Experimental oncology - 1 Sept 2005
Malanchuk O M, Pozur V, Panasyuk G G, Nemazanyy I O, Filonenko V V, Gout I T, Palchevskyy S S
Abstract excerpt
AIM: To identify novel tuberous sclerosis complex (TSC2) binding partners by yeast two-hybrid screening. METHODS: The yeast two-hybrid system DupLEX-A developed by OriGene Technologies and Mouse embryo and HeLa cells cDNA libraries were used in this study. The "bait" constructs, containing full-length and truncated form of TSC2 were prepared. The expression of all constructs in yeast was confirmed by...
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