Article
[The acquisition of two silkworm CAPs markers and their use in genetic diversity and phylogenetic relationship].
Yi chuan = Hereditas - 1 Jul 2005
Huang Jian-Hua, Miao Xue-Xia, Li Mu-Wang, Zhang Yong, Zhao Wei-Guo, Huang Yong-Ping
Abstract excerpt
Cleaved amplified polymorphic sequence (CAPs) markers are based on PCR amplification of known genes, cDNA sequences or RAPD sequences. The PCR products are digested by restriction enzymes, generating the simple type of data as heterozygotes and homozygotes. Here we designed primers based on silkworm attacin and alpha-amylase genes, then digested the PCR products in silkworm strains P50, C108 and their progeny F1...
Topics
- Amplified Fragment Length Polymorphism Analysis
- Animals
- Base Sequence
- Bombyx
- DNA Primers
- Genetic Markers
- Genetic Variation
- Insect Proteins
- Molecular Sequence Data
- Phylogeny
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Sequence Homology, Nucleic Acid
- alpha-Amylases
