Article
Identification of genes involved in cytochrome c biogenesis in Shewanella oneidensis, using a modified mariner transposon.
Applied and environmental microbiology - 1 Aug 2005
Bouhenni R, Gehrke A, Saffarini D
Abstract excerpt
A modified mariner transposon, miniHimar RB1, was generated to mutagenize cells of the metal-reducing bacterium Shewanella oneidensis. The use of this transposon led to the isolation of stable mutants and allowed rapid identification of disrupted genes. Fifty-eight mutants, including BG104 and BG148 with transposon insertions in the cytochrome c maturation genes ccmC and ccmF1, respectively, were analyzed. Both...
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