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Article

Genetic diversity of viable, injured, and dead fecal bacteria assessed by fluorescence-activated cell sorting and 16S rRNA gene analysis.

Applied and environmental microbiology - 1 Aug 2005

Ben-Amor Kaouther, Heilig Hans, Smidt Hauke, Vaughan Elaine E, Abee Tjakko, de Vos Willem M

Abstract excerpt

A novel approach combining a flow cytometric in situ viability assay with 16S rRNA gene analysis was used to study the relationship between diversity and activity of the fecal microbiota. Simultaneous staining with propidium iodide (PI) and SYTO BC provided clear discrimination between intact cells (49%), injured or damaged cells (19%), and dead cells (32%). The three subpopulations were sorted and characterized...

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Identifiers

PMID: 16085863
DOI: 10.1128/AEM.71.8.4679-4689.2005
PMCID: PMC1183343

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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