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Article

Effects of methyl‐β‐cyclodextrin‐mediated cholesterol depletion in porcine sperm compared to somatic cells

26 Jul 2005

Abstract excerpt

In this study, the use of methyl-beta-cyclodextrin (MBCD) to support capacitation of sperm cells was studied. Sperm were incubated with MBCD or alternatively capacitated in an in vitro fertilization medium. The effects of these incubations on phospholipid scrambling (using merocyanin), cholesterol depletion, GM-1 localization (using cholera-toxin B (CTX)), and membrane deterioration were assessed. For comparison,...

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PMID: 16044473
DOI: 10.1002/mrd.20351

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