Article
Ca2+-related changes in the capacitation state of human spermatozoa assessed by a chlortetracycline fluorescence assay
1 Sept 1993
Abstract excerpt
Chlortetracycline (CTC) fluorescence patterns were used to assess Ca -related changes in the capacitation state of human spermatozoa incubated under conditions that would affect their intracellular Ca2+ levels. Initial experiments were designed to identify consistently occurring patterns and to correlate these with acrosomal status. Incubation for up to 1 h with the ionophore A23187 (10 μmol l−1), known to...
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