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Coccidioidomycosis and blastomycosis: Advances in molecular diagnosis

18 Jul 2005

Abstract excerpt

Clinical isolates of Coccidioides spp. and Blastomyces dermatitidis can be identified by chemiluminescent DNA probes and PCR assays targeting multicopy genes. In fixed tissue samples, cells of the two fungi are specified by in situ hybridization and PCR assays targeting 18S rDNA but sequencing of the products is mandatory. Nested PCR assays targeting genes encoding species- or genus-specific proteins like proline...

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PMID: 16043334
DOI: 10.1016/j.femsim.2005.05.011

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