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Real-Time PCR Assay for Identification of Blastomyces dermatitidis in Culture and in Tissue

8 Mar 2012

Abstract excerpt

The TaqMan real-time PCR assay was developed from the Blastomyces dermatitidis BAD1 gene promoter. The assay identified all haplotypes of B. dermatitidis and five of six positive paraffin-embedded tissues. The assay sensitivity threshold was 1 pg genomic DNA of the mold form and 2 CFU of the yeast form of B. dermatitidis. No cross-reactivity was observed against other fungal DNA. The assay allowed rapid (5-h)...

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PMID: 22403418
DOI: 10.1128/jcm.00310-12

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