Article
Analysis of promoters recognized by HrpL, an alternative sigma-factor protein from Pantoea agglomerans pv. gypsophilae.
Molecular plant-microbe interactions : MPMI - 1 Jul 2005
Nissan Gal, Manulis Shulamit, Weinthal Dan M, Sessa Guido, Barash Isaac
Abstract excerpt
HrpL, an alternative sigma factor, activates the transcription of the Hrp regulon by its binding to a common "hrp box" promoter. Based on computational techniques, the hrp box previously was defined as a consensus bipartite cis element, 5'-GGAACC-N(15-16)-CCACNNA-3'. The present report combines a quantitative in vivo assay for measuring Hrp promoter activity with site-specific mutagenesis to analyze the effect of...
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