Article
Detection of mitochondrial DNA from human inner ear using real-time polymerase chain reaction and laser microdissection.
Acta oto-laryngologica - 1 Jul 2005
Kimura Yurika, Kouda Hiroko, Eishi Yoshinobu, Kobayashi Daisuke, Suzuki Yoshimi, Ishige Ikuo, Iino Yukiko, Kitamura Ken
Abstract excerpt
CONCLUSIONS: In this study we were able to amplify and analyze extremely small amounts of template DNA from only a few individually dissected cells. We anticipate that this approach will facilitate the detection and analysis of mitochondrial (mt) DNA mutations in specific cell types in the inner ear, which should shed new light on genetic disorders leading to hearing loss. OBJECTIVE: To isolate mtDNA from...
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