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Rapid detection methods and prevalence estimation for Borrelia lonestari glpQ in Amblyomma americanum (Acari: Ixodidae) pools of unequal size.

Vector borne and zoonotic diseases (Larchmont, N.Y.) - 1 Jan 2005

Bacon Rendi Murphree, Pilgard Mark A, Johnson Barbara J B, Piesman Joseph, Biggerstaff Brad J, Quintana Miguel

Abstract excerpt

DNA was extracted from pools of Amblyomma americanum ticks collected from vegetation at two sites in Fort Leonard Wood, Missouri and tested for the presence of Borrelia spp. Two new methods were developed to detect Borrelia lonestari DNA by targeting the glycerophosphodiester phosphodiesterase (glpQ) gene. The first method detected B. lonestari DNA using a SYBR green I melting curve analysis of the PCR product...

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PMID: 16011431
DOI: 10.1089/vbz.2005.5.146

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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