Detection and separation of Rhizobium and Bradyrhizobium Nod metabolites using thin-layer chromatography.

doi:10.1094/mpmi-5-072

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Article

Detection and separation of Rhizobium and Bradyrhizobium Nod metabolites using thin-layer chromatography.

Molecular plant-microbe interactions : MPMI - 1 Jan 2000

Spaink H P, Aarts A, Stacey G, Bloemberg G V, Lugtenberg B J, Kennedy E P

Abstract excerpt

Using radioactive acetate as a precursor, it was shown that the common nodABC genes of Rhizobium and Bradyrhizobium strains are involved in the production of one or more metabolites that are excreted into the growth medium. A rapid thin-layer chromatography (TLC) system has been developed to sepa...

Topics

Acetates
Acetyltransferases
Acyltransferases
Amidohydrolases
Bacterial Proteins
Genetic Variation
Membrane Proteins
N-Acetylglucosaminyltransferases
Rhizobiaceae
Symbiosis

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