Article
Detection and separation of Rhizobium and Bradyrhizobium Nod metabolites using thin-layer chromatography.
Molecular plant-microbe interactions : MPMI - 1 Jan 2000
Spaink H P, Aarts A, Stacey G, Bloemberg G V, Lugtenberg B J, Kennedy E P
Abstract excerpt
Using radioactive acetate as a precursor, it was shown that the common nodABC genes of Rhizobium and Bradyrhizobium strains are involved in the production of one or more metabolites that are excreted into the growth medium. A rapid thin-layer chromatography (TLC) system has been developed to separate these so-called Nod metabolites that can then be visualized by autoradiography. Different patterns of Nod...
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