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Article

Necrotizing activity of five Botrytis cinerea endopolygalacturonases produced in Pichia pastoris.

The Plant journal : for cell and molecular biology - 1 Jul 2005

Kars Ilona, Krooshof Geja H, Wagemakers Lia, Joosten Rob, Benen Jacques A E, van Kan Jan A L

Abstract excerpt

Five Botrytis cinerea endopolygalacturonase enzymes (BcPGs) were individually expressed in Pichia pastoris, purified to homogeneity and biochemically characterized. While the pH optima of the five enzymes were similar (approximately pH 4.5) the maximum activity of individual enzymes differed significantly. For hydrolysis of polygalacturonic acid (PGA), the V(max,app) ranged from 10 to 900 U mg(-1), while the...

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PMID: 15998308
DOI: 10.1111/j.1365-313X.2005.02436.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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