Characterization of the replication region of plasmid pLS32 from the Natto strain of Bacillus subtilis.

doi:10.1128/JB.187.13.4315-4326.2005

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Article

Characterization of the replication region of plasmid pLS32 from the Natto strain of Bacillus subtilis.

Journal of bacteriology - 1 Jul 2005

Tanaka Teruo, Ishida Hirofumi, Maehara Tomoko

Abstract excerpt

Plasmid pL32 from the Natto strain of Bacillus subtilis belongs to a group of low-copy-number plasmids in gram-positive bacteria that replicate via a theta mechanism of replication. We studied the DNA region encoding the replication protein, RepN, of pLS32, and obtained the following results. Transcription of the repN gene starts 167 nucleotides upstream from the translational start site of repN. The copy number...

Topics

Alanine
Bacillus subtilis
Bacterial Proteins
Base Sequence
DNA Replication
Genes, Bacterial
Histidine
Molecular Sequence Data
Mutation
Plasmids
Protein Binding
Replicon
Tandem Repeat Sequences
Threonine
Transcription Initiation Site
Transcription, Genetic

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Identifiers

PMID: 15968040
DOI: 10.1128/JB.187.13.4315-4326.2005
PMCID: PMC1151765

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