Article
Sequence-based typing of the HLA-A10/A19 group and confirmation of a pseudogene coamplified with A*3401.
Human immunology - 1 May 2005
Swelsen Wendy T N, Voorter Christina E M, van den Berg-Loonen Ella M
Abstract excerpt
The strategy for sequencing human leukocyte antigen (HLA)-A was based on separate amplification of exons 2 and 3, followed by forward and reverse heterozygous sequencing of the alleles. Validation of the method was obtained by sequencing 11 individuals carrying alleles from all different HLA-A allele groups, except *43. All alleles could be correctly identified except A*3401. Unexpected polymorphic positions were...
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