Sequence-based typing of the HLA-A10/A19 group and confirmation of a pseudogene coamplified with A*3401.

doi:10.1016/j.humimm.2005.01.005

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Article

Sequence-based typing of the HLA-A10/A19 group and confirmation of a pseudogene coamplified with A*3401.

Human immunology - 1 May 2005

Swelsen Wendy T N, Voorter Christina E M, van den Berg-Loonen Ella M

Abstract excerpt

The strategy for sequencing human leukocyte antigen (HLA)-A was based on separate amplification of exons 2 and 3, followed by forward and reverse heterozygous sequencing of the alleles. Validation of the method was obtained by sequencing 11 individuals carrying alleles from all different HLA-A al...

Topics

Alleles
Exons
Genetic Variation
HLA-A Antigens
Heterozygote
Histocompatibility Testing
Homozygote
Humans
Introns
Polymerase Chain Reaction
Pseudogenes
Sequence Analysis, DNA

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